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miR-1260b acts as a negative regulator of adipogenic differentiation. (A) qRT-PCR analysis validating the transfection efficiency, showing robust overexpression (OE) of miR‑1260b in hASCs compared with the normal control (NC). (B) Representative images of Oil Red O staining in hASCs transfected with NC, miR‑1260b mimic, or miR‑1260b inhibitor during adipogenesis. Scale bars represent 100 µm. (C, D) qRT-PCR (C) and Western Blot (D) analyses of key adipogenic markers ( PPARG , ADIPOQ , and FABP4 ) in the three groups. Data represent the mean ± SD. Significance was determined by unpaired two-tailed Student’s t test analysis. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Adipocyte

Article Title: Super-enhancer-associated miR-1260b coordinates adipogenesis and metabolic programming in human adipose stem cells

doi: 10.1080/21623945.2026.2638071

Figure Lengend Snippet: miR-1260b acts as a negative regulator of adipogenic differentiation. (A) qRT-PCR analysis validating the transfection efficiency, showing robust overexpression (OE) of miR‑1260b in hASCs compared with the normal control (NC). (B) Representative images of Oil Red O staining in hASCs transfected with NC, miR‑1260b mimic, or miR‑1260b inhibitor during adipogenesis. Scale bars represent 100 µm. (C, D) qRT-PCR (C) and Western Blot (D) analyses of key adipogenic markers ( PPARG , ADIPOQ , and FABP4 ) in the three groups. Data represent the mean ± SD. Significance was determined by unpaired two-tailed Student’s t test analysis. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The primary antibodies used were against PPARG (Cat: #2430S, Cell Signalling), ADIPOQ (Cat: #2789S, Cell Signalling), FABP4 (Cat: #50699S, Cell Signalling), and GAPDH (Cat: #10494, Proteintech).

Techniques: Quantitative RT-PCR, Transfection, Over Expression, Control, Staining, Western Blot, Two Tailed Test